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Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology) (Methods in Molecular Biology) | 
 enlarge | Creators: Gary D. Foster, Sally Taylor
Publisher: Humana Press
Category: Book
List Price: $104.50
Buy New: $78.28
You Save: $26.22 (25%)
New (10) Used (7) from $78.28
Sales Rank: 976160
Media: Hardcover
Edition: 1
Number Of Items: 1
Pages: 592
Shipping Weight (lbs): 2.1
Dimensions (in): 9.3 x 6.4 x 1.3
ISBN: 0896033856
Dewey Decimal Number: 579.28
EAN: 9780896033856
ASIN: 0896033856
Publication Date: February 24, 1998
Availability: Usually ships in 1-2 business days
Shipping: International shipping available
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Product Description
A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.
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